If you have any question concerning the suitability of your samples, please feel free to contact: .
General recommendation: The use of buffers containing glycerol, DTT or chaotropic agents should be avoided or minimized, as they can alter critical experimental parameters. If your sample requires reducing agent, we recommend TCEP at 0.5 mM.
For hydrodynamic characterization:
- AUC: 1 mL of samples at concentrations between 0.5 and 1 mg/mL is sufficient for most analyses.
- MALLS/viscometry: A typical measurement requires a volume of about 100 µL of protein at a concentration between 0.1 and 1 mg/mL.
- TD/viscometry: A typical measurement requires a protein concentration between 0.1 and 1 mg/mL. Note that his technique can also be used to measure the viscosity of your buffers.
- DLS: We use cells with a loading volume of 30 µL. A typical protein concentration for QC goes from 0.1 to 1 mg/mL. We recommend you estimate the required protein concentration using the following formula:[conc]= 15/MW where [conc]and MW and are respectively in mg/mL and kDa.
- Densitometry: Generally used to measure the density of buffers. Exceptionally it can be used to measure partial specific volumes of samples.
For more information, please feel free to address your questions to: .
For kinetic characterization:
- SPR and BLI: For the immobilized interaction partner, 10 to 100µg (at a concentration of at least 100 µg/ml) are typically required depending on the chosen immobilization strategy (covalent or non-covalent). For the interaction partner in solution, a minimal volume of 200 µl is required, at a molar concentration 100 times higher than the estimated Kd.
For more information, please feel free to address your questions to: .
For quality control and optimization:
A typical protein concentration for QC goes from 0.1 to 1 mg/mL for a volume about 100 µL. You can estimate the required protein concentration using the formula: [conc]= 15/MW ([conc] in mg/mL and MW in kDa)