3) ULB – Centre for Structural Biology and Bioinformatics, Université Libre de Bruxelles

Location: Brussels, Belgium
Web-site(s):

MOSBRI reference site for the following techniques:
FTIR: Fourier Transform Infrared Spectroscopy

Partner description

The infrastructure is located in the Centre for Structural Biology and Bioinformatics of the Université Libre de Bruxelles, in Brussels. Combining protein microarrays with state-of-the-art FTIR imaging is a unique tool. It provides high throughput analysis of proteins.

Instruments / methodologies offered through TNA

The main pieces of equipment are:

  • Microarrayer: protein microarrays (up to 4,000 spots/cm2) are produced by an Arrayjet Marathon Microarrayer (ArrayJet, Roslin, UK). Temperature, relative humidity  and HEPA air filtration control provided by a Jetmosphere TM Environmental Control System (ArrayJet, Roslin, UK).
  • Pipetting robot: Pipetting robot Freedom EVO 150 (AirLiha 8) robotic unit from TECAN. Used to prepare 384 well-paltes to feed the microarrayer.
  • FTIR imaging: data are collected using an Agilent 128 x 128 Mercury Cadmium Telluride (MCT) Focal Plane Array (FPA) detector for mid-IR imaging. 15x and 4x objectives + high magnification.
  • Standard FTIR spectroscopy and accessories: five FTIR spectrometers with MCT detectors, ATR, variable angle ATR, polarizers, high throughput 96-well plates transmission FTIR spectroscopy.

Access modality: Typically 3-5 days are sufficient for an exhaustive study of a few samples. Both physical and remote access is provided.

Sample requirements

Full details of the sample requirements for the techniques offered at this TNA site can be found here.