AUC: Analytical Ultra Centrifugation

Analytical Ultra Centrifugation combines an ultracentrifuge with optical absorbance measurements and monitors a sample sedimentation profile over time. This gives information on the samples overall shape, molar mass and size distribution.

What are the sample requirements?

  • 1 mL of samples at concentrations between 0.5 and 1 mg/mL is sufficient for most analyses.

What other specific considerations are relevant?

  • The technique relies heavily on selective absorbance measurements to monitor sedimentation effects so high levels of purity is not an absolute concern. However, this does become a factor of consideration if a specific absorbance wavelength is not selectable for the target protein in an impure mixture.

Partners offering this technique

MOSBRI reference partner site for this technique:

Other partners: