An in-house built continuous flow (original prototype) based on a capillary mixing methodology with a fluorescence CCD camera is available at DSB-UROM. In a continuous flow apparatus, two separated solutions are continuously mixed into a chamber where a spectroscopic signal change is recorded. Absorbance and fluorescence signals can be detected. The signal change occurring upon solutions mixing is monitored at different positions downstream from the mixing point and converted into a time-dependent signal change on the basis of the measured flow rate. This methodology can resolve much faster reaction kinetics compared to stopped-flow, thanks to a shorter dead-time (50µs).
Generally suitable to monitor fast kinetics of reactions involving protein-protein interactions, protein (un)folding, enzyme activity, ligand binding, Forster resonance energy transfer (FRET).
Sample requirements:
- Pure protein samples are strongly suggested
- Protein concentrations may vary for different types of experiments and should be previously discussed with the Facility team
Partners offering this technique
MOSBRI reference partner site for this technique: