Electron Paramagnetic Resonance (EPR) is the method of choice to study samples with unpaired electrons: radicals, transition metal ions, lanthanides, etc… Diamagnetic samples do not exhibit any EPR signal. EPR measures the absorption of microwave radiation by a sample as a function of the magnetic field applied to the sample. Apart from a conventional EPR spectrum, many other experiments can be performed allowing different applications in the fields of catalysis, protein dynamics, and structural biology.
What are the sample requirements?
- Pure samples are requested.
- For a Spin Labelling (Nitroxide label) experiment, 50-100 nmoles are required.
- cw-EPR: 10-50 µM in 50 µL / pulsed EPR (DEER) 60-100 µM in 15 µL
- For metalloproteins, cw and pulsed EPR: typical concentrations 50-100 µM in 100-150 µL
What other specific considerations are relevant?
- Avoid the presence of paramagnetic species in the buffer (Mn2+, Fe3+, …)
- Indicate the presence of reducing agents (DTT, TCEP, …)
- Please contact for specific details on the experimental protocol
Partners offering this technique
MOSBRI reference partner site for this technique: