Small angle X-ray scattering is used to get information about structure of a sample on the nanoscale as well as morphological information. This includes shape analysis of e.g. proteins, which can be compared to structural models of the sample. The technique allows high-level characterization of particles in solution and assessment of sample quality.
What are the sample requirements?
- Minimal volume: 10-20 μL.
- Sample concentration: 1-10 mg/mL. Typically, concentration series are advised for complete analysis.
- Samples should be both pure and as monodisperse as is possible (preferably above 95%)
What other specific considerations are relevant?
- Blank buffer has to be exactly the same as the sample buffer. Dialysis is suggested as the last step of sample preparation.
Partners offering this technique
MOSBRI reference partner site for this technique: