Time-resolved single particle fluorescence is performed with a MicroTime 200 (PicoQuant) instrument and is based on a Time-resolved Confocal Fluorescence Microscope. Both diffusion-based single molecule experiments and fluorescence correlation spectroscopy are possible on fluorophore labelled proteins or other macromolecules. The instrument can be used to sense changes in the local environment of a macromolecule, typically a protein or a protein complex, as well as detecting and characterizing interactions and conformal changes.
What are the sample requirements?
- Highly pure samples are required.
- Standard measurements are performed with 50 μL of 200 pM labelled protein for single-molecule experiments or 1 nM for fluorescence correlation spectroscopy measurements
What other specific considerations are relevant?
- The attachment of an appropriate fluorophore probe to the macromolecule of study is required.
- Please contact for specific details on the experimental protocol
- Contact person: Nunilo Cremades ()
Partners offering this technique
MOSBRI reference partner sites for this technique: