ESC5: Label-free techniques for the characterization of protein interactions

Dates: 3rd-5th May 2023

Location: Institute of Biotechnology of the Czech Academy of Sciences (MoB-IBT)

Full address: Prumyslova 595, 252 50 Vestec, Czech Republic

Number of participants: 10

It is no longer possible to apply for this course.

Description of the course:

The course will be focused on the application of label-free biophysical techniques iTC, MST, and DSF for the characterization of protein interactions and will combine both theoretical and practical content.

Outline of the course:

The course will start on Wednesday 3rd of May afternoon and end on Friday 5th of May afternoon.

Day 1


Lecture 1:       Introduction to iTC: principles and experimental design
Lecture 2:       Principles of MST technique, Label-Free MST, Differential scanning fluorimetry technique

Tour of the Centre of Molecular Structure facilities.

Day 2

Hands-on workshop in two groups of 5 participants.

1st group – iTC
2nd group – LF MST, DSF


1st group – LF MST, DSF
2nd group – iTC

Day 3

Troubleshooting, data analysis, questions.

~ 1.5 hour 1st group – iTC; 2nd group – LF MST, DSF,
next 1.5 hours 1st group – LF MST, DSF; 2nd group – iTC.


Other details:

Participants will receive partial financial support to attend the course, including economy travel, lunches, dinners and 2 nights of accommodation with breakfast (accommodation will be booked by the organizers). Ten travel bursaries of a maximum 300 euros each will be offered to cover travel costs. Successful applicants will be informed of the eligible expenses when they receive a formal acceptance letter.

Download a pdf version of a flyer for this course via the link below:


Application to take part in the course

The course is limited to 10 participants and therefore any interested parties will need to submit an application for consideration to take part.

Who is eligible to apply:

Anyone with a workplace/host institution in one of the EU member or associated states may apply to attend this course (this includes people from the UK). Applicants from industry are also welcome. It is expected that the applicant has at least a basic knowledge of molecular biology, chemistry and/or biophysics and that it is applicable to their area of research/work. Applications from scientists in the early-stage of their career are particularly encouraged and we will strive to obtain a gender balance of the participants in the course.

How to apply:

The application consists of:

  • submission of a completed course application form which includes:
    • motivation for attendance
    • a short scientific CV
  • if you are a student or postdoc, a reference letter.

Once the application period is closed, all submissions will be reviewed and the applicants will be informed of the outcome.

When to apply:

It is no longer possible to apply for this course. The applications received are under review and applicants will be informed once a decision has been made.

Application deadline:

The deadline for submission of an application to participate in this course was the: 12th March 2023.