BLS2: Techniques to study single molecule/particle interactions

Date(s) - 13/05/24 - 17/05/24
All day event


Scientific Organizers:
Zdenek Lansky (Institute of Biotechnology, Czech Academy of Sciences, Prague)
Carsten Janke (Institut Curie, Orsay, France)

Single molecule imaging techniques enable following bio-molecular interactions with unprecedented resolution. Following single molecules in cells is often challenging due to the complex geometry of the intracellular environment. By contrast, in in vitro reconstituted systems, comprising only few bio-molecules of interest, single molecule imaging is achievable e.g. by placing the sample in close vicinity of a glass surface and using Total Internal Reflection Fluorescence (TIRF) microscopy. However, the main obstacle of this method is obtaining functional bio-molecular samples in high enough quantity and purity. During the Basic Level School, we will explore an experimental approach combing the best of the two worlds by imaging bio-molecular interactions with single molecule resolution in cell lysates. This method employs the usage of established in vitro methods, such as TIRF imaging, while not being dependent on laborious sample preparation, and, importantly, enables following single molecule dynamics in the presence of other cellular components.

This MOSBRI basic level school (BLS2) held at MOSBRI partner MoB-IBT. More details about this school, including how to apply, will be added to the website closer to the time.